| 1. | Water quality - detection of human enteroviruses by monolayer plaque assay
水质.用单分子层噬菌斑序列检测人体肠病毒 |
| 2. | Water quality - detection of human enteroviruses by monolayer plaque assay
水质.通过单层噬菌斑检验探测人体肠道病毒 |
| 3. | Water quality - detection of human enteroviruses by monolayer plaque assay ; german version en 14486 : 2005
水质.通过单层噬菌斑检验探测人体肠道病毒 |
| 4. | The recombinant meq - baculovirus was obtained by co - transfecting the insect sf9 cells with pblubac4 - meq and linearised bac - n - blue dna . the recombinant baculovirus was selected by plaque assay and confirmed by pcr technique and sequencing of the inserted gene
应用重组痘病毒表达的meq制备的单抗23b46对重组杆状病毒感染的sfg细胞及其裂解物分别进行间接免疫荧光试验、 westemblot和免疫沉淀试验的检测。 |
| 5. | The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol . to purify the recombinant virus , we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock
构建重组病毒：用已经构建好的angiostatin杆状病毒转移载体pbluebachiszb和病毒dna共同转染sfg细胞，通过蚀斑实验筛选出纯的重组斑点并扩增产生p二病毒贮存液。 |
| 6. | Firstly , a reverse hemolytic plaque assay ( rhpa ) was used to scale testosterone secretion of leydig ' s cells in single cells level . sequentially , star mrna expression was detected with fluorescent in situ hybridization ( fish ) at the same cells
本研究首先采用逆向溶血斑法，鉴定单个间质细胞睾酮分泌上的差异，然后，在被鉴定的同一间质细胞上进行原位杂交( fish ) ，以便在单个细胞水平上确定间质细胞是否存在功能及star基因表达的异质性。 |
| 7. | In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection
此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。 |
| 8. | The homology of recombinant virus bmpak - hbmp was obtained and identified by plaque assay and baculovirus contains the hbmp gene was confirmed through pcr and dna dot blotting . the expressed rhbsag was determined by elisa after infecting bm - n cells and pupae with recombinant virus bmpak - hbmp and bmpak - hbm ( containing nonfusion hbv surface antigen medium sized )
用重组病毒bmpak - hbmp和bmpak - hbm [带有非融合乙肝表面抗原( pres2 + s )基因，为本实验构建]感染家蚕细胞及蛹，对两种病毒的表达产物用elisa进行了跟踪检测，结果表明，感染bmpak - hbmp的家蚕细胞及蛹中rhbsag的表达量分别为3 |
| 9. | After obvious cytopathogenic effects developed , virus - contained supematants were harvested , and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal . single blue plaques were picked , and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification . the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
该载体与具有高度感染性的bartha - k61株基因组dna通过脂质体加plus法共转染vero细胞，采用甲基纤维素固定病变， x - gal染色，经过10代蓝斑纯化获得了一株稳定表达lacz基因的ge tk基因缺失突变株，命名为rprv - lacz 。 |
